To be successful in cell culture, it is essential to remain a contamination free environment (bacteria, fungi etc) Aspetic techniques ensure that no microorganisms enter the cell culture. Cell culture sterility is ensured by set of procedures.
Aseptic techniques required while working with cell culture.
| Handling | Reagents/Media | Workplace |
| Slow/careful handling. | Pre-sterilisation of all reagents/equipment | Cell culture hood works properly |
| Sterilization of all items before starting. | No contamination in reagents (expiration date, appearance normal). | Frequent de-contamination (hood, fridge etc) |
| Sterile pipettes | Work area: sterile and tidy | |
| No touching of sterile items to non-sterilized surfaces |