7. Transport in Cells

Facilitated Diffusion

Water and many other substances cannot simply diffuse across a membrane. Hydrophilic molecules, charged ions, and relatively large molecules such as glucose all need help with diffusion. The help comes from special proteins in the membrane known as transport proteins. Diffusion with the help of transport proteins is called facilitated diffusion. There are several types of transport proteins, including channel proteins and carrier proteins (Figure

  • Channel proteins form pores, or tiny holes, in the membrane. This allows water molecules and small ions to pass through the membrane without coming into contact with the hydrophobic tails of the lipid molecules in the interior of the membrane.
  • Carrier proteins bind with specific ions or molecules, and in doing so, they change shape. As carrier proteins change shape, they carry the ions or molecules across the membrane.
Scheme facilitated diffusion in cell membrane
Figure Diffusion Across a Cell Membrane. Channel proteins and carrier proteins help substances diffuse across a cell membrane. In this diagram, the channel and carrier proteins are helping substances move into the cell (from the extracellular space to the intracellular space). The channel protein has an opening that allows the substances to cross. In a carrier protein, the substance binds to the protein, which then causes the protein to changes shape, thereby releasing the substance into the cell.
7. Transport in Cells


Osmosis is a specific type of diffusion; it is the passage of water from a region of high water concentration through a semi-permeable membrane to a region of low water concentration. Water moves in or out of a cell until its concentration is the same on both sides of the plasma membrane.

Semi-permeable membranes are very thin layers of material that allow some things to pass through them but prevent other things from passing through. Cell membranes are an example of semi-permeable membranes. Cell membranes allow small molecules such as oxygen, water carbon dioxide, and oxygen to pass through but do not allow larger molecules like glucose, sucrose, proteins, and starch to enter the cell directly.

The classic example used to demonstrate osmosis and osmotic pressure is to immerse cells into sugar solutions of various concentrations. There are three possible relationships that cells can encounter when placed into a sugar solution. Figure shows what happens in osmosis through the semi-permeable membrane of the cells.

  1. The concentration of solute in the solution can be greater than the concentration of solute in the cells. This cell is described as being in a hypertonic solution (hyper = greater than normal). The net flow or water will be out of the cell.
  2. The concentration of solute in the solution can be equal to the concentration of solute in cells. In this situation, the cell is in an isotonic solution (iso = equal or the same as normal). The amount of water entering the cell is the same as the amount leaving the cell.
  3. The concentration of solute in the solution can be less than the concentration of solute in the cells. This cell is in a hypotonic solution (hypo = less than normal). The net flow of water will be into the cell.
water moves out of the cell. inside has 20% solutes (80% water). outside has 40% solutes (60% water).
Figure 5.7.4.A5.7.4.A: Hypertonic solution. A solution that has a higher solute concentration than another solution. Water particles will move out of the cell, causing crenation.
water moves equally into and out of the cell due to the same concentration of solutes inside and out (in this case, 20% solutes and 80% water)
Figure 5.7.4.B5.7.4.B: Isotonic solution. A solution that has the same solute concentration as another solution. There is no net movement of water particles, and the overall concentration on both sides of the cell membrane remains constant.
water moves into the cell.  inside has 20% solutes (80% water). outside has 10% solutes (90% water).
Figure 5.7.4.C5.7.4.C: Hypotonic solution. A solution that has a lower solute concentration than another solution. Water particles will move into the cell, causing the cell to expand and eventually lyse.

Figure demonstrates the specific outcomes of osmosis in red blood cells.

  1. Hypertonic solution. The red blood cell will appear to shrink as the water flows out of the cell and into the surrounding environment.
  2. Isotonic solution. The red blood cell will retain its normal shape in this environment as the amount of water entering the cell is the same as the amount leaving the cell.
  3. Hypotonic solution. The red blood cell in this environment will become visibly swollen and potentially rupture as water rushes into the cell.
Osmotic pressure on blood cells
Figure Osmosis demonstration with Red Blood cells places in a hypertonic, isotonic, and hypotonic solution.
7. Transport in Cells

Simple Diffusion

Diffusion Although you may not know what diffusion is, you have experienced the process. Can you remember walking into the front door of your home and smelling a pleasant aroma coming from the kitchen? It was the diffusion of particles from the kitchen to the front door of the house that allowed you to detect the odors. Diffusion is defined as the net movement of particles from an area of greater concentration to an area of lesser concentration.

Scheme of simple diffusion through cell membrane
Simple diffusion shows as a timeline with the outside of the cell (extracellular space) separated from the inside of the cell (intracellular space) by the cell membrane. In the beginning of the timeline there are many molecules outside of the cell and none inside. Over time, they diffuse into the cell until there is an equal amount outside and inside.

The molecules in a gas, a liquid, or a solid are in constant motion due to their kinetic energy. Molecules are in constant movement and collide with each other. These collisions cause the molecules to move in random directions. Over time, however, more molecules will be propelled into the less concentrated area. Thus, the net movement of molecules is always from more tightly packed areas to less tightly packed areas. Many things can diffuse.

Odors diffuse through the air, salt diffuses through water and nutrients diffuse from the blood to the body tissues. This spread of particles through the random motion from an area of high concentration to an area of lower concentration is known as diffusion. This unequal distribution of molecules is called a concentration gradient.

Once the molecules become uniformly distributed, a dynamic equilibrium exists. The equilibrium is said to be dynamic because molecules continue to move, but despite this change, there is no net change in concentration over time. Both living and nonliving systems experience the process of diffusion. In living systems, diffusion is responsible for the movement of a large number of substances, such as gases and small uncharged molecules, into and out of cells.

7. Transport in Cells

Transport Without Energy

Passive transport occurs when substances cross the plasma membrane without any input of energy from the cell. No energy is needed because the substances are moving from an area where they have a higher concentration to an area where they have a lower concentration. Water solutions are very important in biology. When water is mixed with other molecules this mixture is called a solution. Water is the solvent and the dissolved substance is the solute. A solution is characterized by the solute. For example, water and sugar would be characterized as a sugar solution. More the particles of a solute in a given volume, the higher the concentration. The particles of solute always move from an area where it is more concentrated to an area where it is less concentrated. It’s a little like a ball rolling down a hill. It goes by itself without any input of extra energy.

The different categories of cell transport are outlined in Figure Cell transport can be classified as follows:

  • Passive Transport which includes
    • Simple Diffusion
    • Osmosis
    • Facilitated Diffusion
  • Active Transport can involve either a pump or a vesicle
    • Pump Transport can be
      • primary
      • secondary
    • Vesicle Transport can involve
      • Exocytosis
      • Endocytosis which includes
        • Pinocytosis
        • Phagocytosis
        • Receptor-Mediated Endocytosis
Cell Transport
The Cell Transport Concept Map illustrates various types of cell transports that happen at the plasma membrane
7. Transport in Cells

Transport Across Membranes

If a cell were a house, the plasma membrane would be walls with windows and doors. Moving things in and out of the cell is an important role of the plasma membrane. It controls everything that enters and leaves the cell. There are two basic ways that substances can cross the plasma membrane: passive transport, which requires no energy; and active transport, which requires energy. Passive transport is explained in this section and Active transport is explained in the next section, Active Transport and Homeostasis.

7. Transport in Cells

Cell Transport