5. Cell Culture

Cell culture environment

Cell culture is an amazing tool that allows for easy control and manipulation of all physiochemical and physiological cell factors, such as, temperature, osmotic pressure, pH, gas, hormones, and nutrients.

Cell culture environment.

Contains nutrients, growth factors, and hormonesAverage pH for mammatian cells is pH 7.4Depends on body temperature of the hostControlled by media
Sera source of growth, tipids,hormones Mammalian cell lines 36-37°COrganic or C02 bicarbonate buffer systems are popular
  Insert cell lines 27-30°CCan impact pH
   4-10% C02 is most common

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