Cell culture is an amazing tool that allows for easy control and manipulation of all physiochemical and physiological cell factors, such as, temperature, osmotic pressure, pH, gas, hormones, and nutrients.
Cell culture environment.
| Media | pH | Temperature | CO2 |
| Contains nutrients, growth factors, and hormones | Average pH for mammatian cells is pH 7.4 | Depends on body temperature of the host | Controlled by media |
| Sera source of growth, tipids,hormones | Mammalian cell lines 36-37°C | Organic or C02 bicarbonate buffer systems are popular | |
| Insert cell lines 27-30°C | Can impact pH | ||
| 4-10% C02 is most common |